Mice were first anesthetized with a 100 mg ketamine/kg body weight (Ketaset) and 10 mg xylazine/kg body weight (Rompun) cocktail, followed by intratracheal administration of single dose of 70 μl of CLLs (Liposoma) at 48 hours before infection. Control mice were administered with PBLs (70 μl per mouse) (Liposoma).

巨噬細(xì)胞清除劑清除肺泡巨噬細(xì)胞流式驗(yàn)證清除效果：

flow cytometry plots showing CLL-mediated depletion of AMs in control and Trpv1DTA mice. PBL was used for control group of mice. CLL or PBL (70 μl per mouse) was inoculated via intratracheal route at 48 hours before the CRKP infection.

品牌：Liposoma

產(chǎn)地：荷蘭

貨號(hào)：CP-005-005

規(guī)格：5ml+5ml

品名：Clodronateliposomes & Control Liposomes

代理：靶點(diǎn)科技

部位：肺臟

細(xì)胞：肺泡巨噬細(xì)胞（AM）

劑量：70ul

方式：滴鼻（intratracheal administration）

時(shí)間點(diǎn)：建模前48h

題目：Lung-innervating nociceptor sensory neurons promote pneumonic sepsis during carbapenem-resistant Klebsiella pneumoniae lung infection